SRP19 Antibody

• Catalog Number: orb1256796
• Category: Antibodies
• Description: SRP19 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SRP19 (NP_003126.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 16kDa
• Target: SRP19
• UniProt ID: P09132
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: SRP19,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SRP19 antibody (orb1256796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat ovary using SRP19 antibody (orb1256796) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using SRP19 antibody (orb1256796) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using SRP19 antibody (orb1256796) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using SRP19 antibody (orb1256796) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human vermiform appendix using SRP19 antibody (orb1256796) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using SRP19 antibody (orb1256796) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using SRP19 antibody (orb1256796). Blue: DAPI for nuclear staining.