SRGAP2 Antibody

• Catalog Number: orb1248922
• Category: Antibodies
• Description: SRGAP2 Antibody
• Target: SRGAP2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-KQEDRQTPRSPDST
• UniProt ID: O75044
• Tested applications: ELISA, IF, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 124kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121kDa according to human NP_056141.2 but the rat protein sequence is stlll provisional). Recommended concentration: 0.1-0.3ug/ml.Immunofluorescence: Transfected HEK293 transiently expressing GFP-tagged Human srGAP2 with the primary antibody stained in the red channel (Confocal IF).
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SRGAP2, SLIT-ROBO Rho GTPase activating protein 2,
• Note: For research use only
• NCBI: NP_056141.2

HEK293 overexpressing Human srGAP2 and probed with orb1248922 at 2.5 ug/ml in the right panel.

orb1248922 (0.1 ug/ml) staining of Rat Cortical Neuron lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.