SREBP1 Antibody

• Catalog Number: orb2643813
• Category: Antibodies
• Description: The low density lipoprotein (LDL) receptor mediates the endocytic uptake of cholesterol-carrying lipoproteins, thereby controlling cholesterol levels in cells and plasma. Transcription of the LDL receptor gene is controlled by a ten base pair sequence in the 5' flanking region, designated sterol regulatory element 1 (SRE-1). When cellular sterol stores are depleted, the element is activated, the gene is transcribed and the cellular uptake of LDL increases. A set of SREbinding proteins (SREBPs) have been identified, including two basic helixloop-helix-leucine zipper (bHLH-zip) transcription factors, designated SREBP-1 and SREBP-2. SREBP-1 and SREBP-2 have been shown to have the same specificity for SRE-1 in vitro and to activate the transcription of reporter genes containing SRE-1 in the same way.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SREBP1/1578
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A recombinant full-length human protein was used as the immunogen for this SREBP1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This SREBP1 antibody is available for research use only.
• UniProt ID: P36956
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free SREBP1 antibody (clone SREBP1/1578) as confirmation of integrity and purity.

IHC staining of FFPE human ovarian carcinoma with SREBP1 antibody (clone SREBP1/1578). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding.