SRCAP Antibody

• Catalog Number: orb1249441
• Category: Antibodies
• Description: SRCAP Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DEAQNIKNFKSQR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Not yet tested - our routine western blotting protocol does not allow for the detection of proteins > 250kDa (calculated size of approx. 344kDa according to NP_006653.2).Immunofluorescence: Strong expression of the protein seen in the Nucleus of A31 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SRCAP
• UniProt ID: Q6ZRS2
• NCBI: NP_006653.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SRCAP, Snf2-related CREBBP activator protein, DOMO
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Not yet tested - our routine western blotting protocol does not allow for the detection of proteins > 250kDa (calculated size of approx. 344kDa according to NP_006653.2).Immunofluorescence: Strong expression of the protein seen in the Nucleus of A31 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249441 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue).