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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Immobilized Human CD58, Fc Tag at 5 ug/mL (100 uL/well) can bind Human CD2, His Tag with a linear range of 0.02-0.313 ug/mL. |
| Target | CD2 |
| Tag | His Tag |
| MW | 22.7 kDa |
| Purity | >98% as determined by SDS-PAGE. |
| Protein Sequence | Lys 25 - Asp 209 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
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The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 22.6 kDa after removal of the signal peptide. The apparent molecular mass of CD2-His is approximately 25-55 kDa due to glycosylation.
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Human CD2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Protocol Information
SRBC Recombinant Protein (orb1228155)
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