SQSTM1 Antibody

• Catalog Number: orb1538372
• Category: Antibodies
• Description: SQSTM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A 14 AA synthetic peptide near the C-Terminus of human SQSTM1. The immunogen is located within the last 50 AA of SQSTM1.
• Concentration: 1 mg/ml
• Dilution range: ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
• Conjugation: Unconjugated
• Target: SQSTM1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Alternative names: SQSTM1, A170, ORCA, OSIL, Paget disease of bone 3,
• Note: For research use only
• Application notes: Further information: SQSTM1 antibody can be used for detection of SQSTM1 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human, mouse, and rat samples and Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested. Western Blot:
• Expiration Date: 12 months from date of receipt.

Immunofluorescence of SQSTM1 in Rat Spleen cells with SQSTM1 antibody at 20 ug/ml.

Western blot of SQSTM1 in Human spleen tissue lysate with SQSTM1 antibody at (A) 1 and (B) 2 ug/ml.