SPRR1B Rabbit Polyclonal Antibody

SKU: orb585691

Description

Rabbit polyclonal antibody to SPRR1B

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Porcine, Rabbit, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Target: SPRR1B
• Molecular Weight: 10kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

SPRR1, GADD33, SPR-IB, CORNIFIN

Application: IHC, Species+tissue/cell type: Mouse eyeball section, Primary antibody dilution: 1:50, Secondary Antibody: Goat anti-rabbit-AlexaFluor 488, Secondary antibody dilution: 1:50.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-SPRR1B Antibody, Titration: 1.0 ug/ml, Positive Control: Jurkat Whole Cell.

Quick Database Links

Gene Symbol

SPRR1B

UniProt

P22528

RefSeq (Protein):NP_003116