Sprouty-4 antibody

• Catalog Number: orb345482
• Category: Antibodies
• Description: Sprouty-4 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 275-299 of Human Sprouty-4 protein.
• Concentration: 1.1 mg/mL
• Dilution range: ELISA: 1:3,000 - 1:12,000, IHC: 1 µg/ml to 10 µg/ml, WB: 1:100 - 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human Sprouty-4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity is expected with Sprouty-4.
• Conjugation: Unconjugated
• UniProt ID: Q9C004
• NCBI: NP_001120968.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit anti-Sprouty4 antibody, Rabbit anti-Sprouty
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band (maybe a doublet) ~35 kDa in size corresponding to Sprouty-4 by western blotting in the appropriate cell lysate or extract. Some non-specific banding may be observed using this antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human heart tissue using Sprouty-4 antibody

Immunohistochemical staining of human liver tissue using Sprouty-4 antibody

Western blot analysis of Lane 1: HeLa. Lane 2: SW13. Lane 3: C2C12. Load: 30 ug per lane using Sprouty-4 antibody