Spred3 Antibody

• Catalog Number: orb1238899
• Category: Antibodies
• Description: Spred3 Antibody
• Target: SPRED3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: Spred3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: Spred3 Antibody is affinity chromatography purified via peptide column.
• Immunogen: Spred3 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3.The immunogen is located within amino acids 140 - 190 of Spred3.
• UniProt ID: Q2MJR0
• MW: Predicted: 45 kDa Observed: 45 kDa
• Tested applications: ELISA, IF, IHC-P, WB
• Application notes: Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Spred3 Antibody: Eve-3, spred-3, EVE-3, Sprouty-re
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_001035987

Western blot analysis of Spred3 in mouse brain tissue lysate with Spred3 antibody at (A) 2 and (B) 4 µg/mL.

Immunohistochemistry of Spred3 in human brain tissue with Spred3 antibody at 2.5 µg/mL.

Immunofluorescence of spred3 in human brain tissue with spred3 antibody at 20 µg/mL.