SPRED3 Antibody

• Catalog Number: orb1238899
• Category: Antibodies
• Description: SPRED3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Spred3 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3.The immunogen is located within amino acids 140 - 190 of Spred3.
• Concentration: 1 mg/mL
• Dilution range: Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 45 kDa Observed: 45 kDa
• Target: SPRED3
• UniProt ID: Q2MJR0
• NCBI: NP_001035987
• Storage: Spred3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Spred3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Spred3 Antibody: Eve-3, spred-3, EVE-3, Sprouty-re
• Note: For research use only
• Application notes: Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Spred3 in mouse brain tissue lysate with Spred3 antibody at (A) 2 and (B) 4 µg/mL.

Immunohistochemistry of Spred3 in human brain tissue with Spred3 antibody at 2.5 µg/mL.

Immunofluorescence of spred3 in human brain tissue with spred3 antibody at 20 µg/mL.