SPRED3 Antibody

SKU: orb1238899

Description

SPRED3 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Spred3 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3.The immunogen is located within amino acids 140 - 190 of Spred3.
• Target: SPRED3
• Molecular Weight: Predicted: 45 kDa Observed: 45 kDa
• Purification: Spred3 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Spred3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Spred3 Antibody: Eve-3, spred-3, EVE-3, Sprouty-related, EVH1 domain-containing protein 3, Spred-3

Western blot analysis of Spred3 in mouse brain tissue lysate with Spred3 antibody at (A) 2 and (B) 4 µg/mL.

Immunohistochemistry of Spred3 in human brain tissue with Spred3 antibody at 2.5 µg/mL.

Immunofluorescence of spred3 in human brain tissue with spred3 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

SPRED3

UniProt

Q2MJR0

RefSeq (Protein):NP_001035987