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Catalog Number | orb402223 |
---|---|
Category | Antibodies |
Description | splicing factor 1/SF1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 68-80 kDa |
UniProt ID | Q15637 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Splicing factor 1; Mammalian branch point-binding Read more... |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A431 cells using anti-splicing factor 1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of splicing factor 1 using anti-splicing factor 1 antibody.Lane 1:human SK-OV-3 cell; 2:human HepG2 cell; 3:mouse NIH/3T3 cell; 4:rat PC-12 cell; 5:mouse RAW264.7 cell.
IF analysis of splicing factor 1 using anti-splicing factor 1 antibody. splicing factor 1 was detected in immunocytochemical section of A431 cells.
IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody.splicing factor 1 was detected in paraffin-embedded section of mouse lung tissue.
IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody.splicing factor 1 was detected in paraffin-embedded section of rat lung tissue.
IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody.splicing factor 1 was detected in paraffin-embedded section of human rectal cancer tissue.
IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody.splicing factor 1 was detected in paraffin-embedded section of mouse small intestine tissue.
IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody.splicing factor 1 was detected in paraffin-embedded section of human placenta tissue.
IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody.splicing factor 1 was detected in paraffin-embedded section of rat small intestine tissue.
FC, ICC, IF, IHC, IHC-Fr, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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