splicing factor 1/SF1 Antibody

• Catalog Number: orb11665
• Category: Antibodies
• Description: splicing factor 1/SF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human splicing factor 1 (11-30aa DFPSKKRKRSRWNQDTMEQK), identical to the related rat and mouse sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat Immunocytochemistry , 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 68330 MW
• UniProt ID: Q15637
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Splicing factor 1;Mammalian branch point-binding p
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: Rat Spleen Tissue Lysate, Lane 2: Rat Liver Tissue Lysate, Lane 3: PANC Cell Lysate, Lane 4: COLO320 Cell Lysate, Lane 5: SW620 Cell Lysate, Lane 6: SKOV Cell Lysate using SF1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Lung Cancer Tissue using SF1 antibody

Confocal immunofluoroscence analysis of formalin-fixed paraffin embedded HELA Cell using SF1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Spleen Tissue using SF1 antibody