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SPESP1 Rabbit Polyclonal Antibody
Catalog Number: orb186200
| Catalog Number | orb186200 |
|---|---|
| Category | Antibodies |
| Description | SPESP1 Rabbit Polyclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IHC-Fr, IHC-P |
| Predicted Reactivity | Rabbit, Rat |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human SPESP1 (151-250/350aa) |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 37 kDa |
| Target | SPESP1 |
| UniProt ID | Q6UW49 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | Equatorial segment protein; ESP; Glycosylated 38 k Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SPESP1) Polyclonal Antibody, Unconjugated (orb186200) at 1:800 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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