SPDYE3 Antibody

SKU: orb1270859

Description

SPDYE3 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Reactivity: Human
• Application Notes:
  For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This SPDYE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 455-483 amino acids from the Central region of human SPDYE3.
• Target: SPDYE3
• Molecular Weight: 64 kDa
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Speedy protein E3, SPDYE3

Western blot analysis in A375 cell line lysates (35 ug/lane).

SPDYE3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Quick Database Links

Gene Symbol

SPDYE3

UniProt

A6NKU9

RefSeq:A6NKU9