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SPC25 Rabbit Polyclonal Antibody

Catalog Number: orb101199

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb101199
CategoryAntibodies
DescriptionSPC25 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P
Predicted ReactivityBovine, Canine, Equine, Human, Porcine, Rabbit, Rat, Sheep
ReactivityMouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human SPC25 (121-224/224aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW26 kDa
TargetSPC25
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesAD024; hSpc25; Kinetochore protein Spc25; SPBC25;
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NoteFor research use only
Expiration Date12 months from date of receipt.
SPC25 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SPC25) Polyclonal Antibody, Unconjugated (orb101199) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

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