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Item 1 of 7
SPAK Antibody (Center)
Catalog Number: orb1928722
| Catalog Number | orb1928722 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB03418 |
| Tested applications | IHC-P, WB |
| Predicted Reactivity | Rat |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 59474 Da |
| Target | This SPAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-376 amino acids from the Central region of human SPAK. |
| UniProt ID | Q9UEW8 |
| NCBI | NP_037365.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | STE20/SPS1-related proline-alanine-rich protein ki Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
SPAK Antibody (A363) western blot analysis in U937 cell line lysates (35 ug/lane).This demonstrates the SPAK antibody detected the SPAK protein (arrow).
Western blot analysis of SPAK (arrow) using rabbit polyclonal SPAK Antibody (A363). 293T cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPAK gene.
Western blot analysis of anti-SPAK Pab in mouse liver tissue lysate. SPAK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of SPAK (arrow) using rabbit polyclonal SPAK Antibody (A363). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPAK gene.
Western blot analysis of lysate from HepG2 cell line, using SPAK Antibody (A363). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
Western blot analysis of lysate from 293 cell line, using SPAK Antibody (A363). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
