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Description
Research Area
Signal Transduction, Spermatogonial stem cells (SSCs), Stem Cell Research
Images & Validation
−
| Tested Applications | ELISA, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Equine, Human, Mouse, Porcine, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human SPAG6 (233-290/509aa) |
| Target | SPAG6 |
| Molecular Weight | 55 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CFAP194; CT141; FAP194; Repro-SA-1; pf16; Spag6; Spag6l1; RGD1310892; XELAEV_18030785mg; PF-16; SPAG6_HUMAN; Protein PF16 homolog; Sperm flagellar protein; SPAG6_MOUSE; Axoneme central apparatus protein; sperm associated antigen 6
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Quick Database Links
Gene Symbol
SPAG6
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)





