SOX5 Antibody

• Catalog Number: orb1254349
• Category: Antibodies
• Description: SOX5 Antibody
• Target: SOX5
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SOX5 (NP_694534.1).
• UniProt ID: P35711
• MW: Observed: 70kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SOX5, L-SOX5, L-SOX5B, L-SOX5F, RP11-444D3.1
• Research Area: Epigenetics & Chromatin
• Note: For research use only

Western blot analysis of extracts of various cell lines, using SOX5 antibody (orb1254349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunohistochemistry of paraffin-embedded rat kidney using SOX5 antibody (orb1254349) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using SOX5 antibody (orb1254349) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using SOX5 antibody (orb1254349) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using SOX5 antibody (orb1254349) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using SOX5 antibody (orb1254349) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using SOX5 antibody (orb1254349).