SOX2 Antibody

• Catalog Number: orb1252216
• Category: Antibodies
• Description: SOX2 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SOX2/1792
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2b, kappa
• Immunogen: Amino acids 176-305 of the human protein were used as the immunogen for the SOX2 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: ELISA: 2-4 ug/ml; order BSA-free formulation for coatingFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTOptimal dilution of the SOX2 antibody should be determined by the researcher.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SOX2
• UniProt ID: P48431
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: SOX2 Antibody: ANOP3, MCOPS3, Transcription factor
• Note: For research use only
• Application notes: ELISA: 2-4 ug/ml; order BSA-free formulation for coatingFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTOptimal dilution of the SOX2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human cervical carcinoma with SOX2 antibody (clone SOX2/1792). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human lung carcinoma with SOX2 antibody (clone SOX2/1792). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of 1) partial human protein and 2) human NCCIT lysate with SOX2 antibody (clone SOX2/1792). Predicted molecular weight ~34 kDa.