SOX10 Antibody

• Catalog Number: orb2637149
• Category: Antibodies
• Description: This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. Expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Often used melanoma markers, such as anti-HMB45 and anti-MelanA, are poorly expressed in desmoplastic melanomas while SOX10 is moderately-to-strongly expressed in desmoplastic melanomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-SOX10-1D8
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: A portion of amino acids 90-179 was used as the immunogen for the SOX10 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This SOX10 antibody is available for research use only.
• UniProt ID: P56693
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the SOX10 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

FACS staining of PFA-fixed human HeLa cells with SOX10 antibody (blue, clone PCRP-SOX10-1D8), and unstained cells (red).

Immunofluorescent staining of PFA-fixed human HeLa cells using SOX10 antibody (green, clone PCRP-SOX10-1D8) and phalloidin (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using SOX10 antibody (clone PCRP-SOX10-1D8). These results demonstrate the foremost specificity of the PCRP-SOX10-1D8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free SOX10 antibody (clone PCRP-SOX10-1D8) as confirmation of integrity and purity.