Somatostatin proprotein (aa58-70)

• Catalog Number: orb668961
• Category: Antibodies
• Description: Goat polyclonal antibody to SST
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:1000, IF: 10 μg/ml
• Conjugation: Unconjugated
• MW: 12.7
• Target: Somatostatin proprotein (aa58-70)
• Entrez: 6750
• Protein Sequence: SEPNQTENDALEP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SST; somatostatin; SMST; growth hormone release-in
• Note: For research use only
• Application notes: Notes: Immunofluorescence: Strong expression of the protein seen in the plasma membrane and endoplasmic reticulum of U2OS and U251 cells. Recommended concentration: 10µg/ml.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane and endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane and endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).