SOD2 Rabbit Polyclonal Antibody

SKU: orb499623

Description

SOD2 Rabbit Polyclonal Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Equine, Mouse, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SOD2 (51-110/222aa)
• Target: SOD2
• Molecular Weight: 22 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MNSOD; SOD2X1; SOD2X2; GC1; GClnc1; IPO-B; IPOB; MVCD6; Mn-SOD; lncRNA-GC1; Sod-2; SODM_BOVIN; SOD2; 1.15.1.1; SODM_HUMAN; SODM_MOUSE; SODM_RABIT; SODM_RAT;

Blank control (blue line): Hep G2 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-SOD2 antibody (orb499623), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SOD 2) Polyclonal Antibody, Unconjugated (orb499623) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

SOD2

UniProt

P04179