SOCS7 Rabbit Polyclonal Antibody

SKU: orb313242

Description

SOCS7 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Cytoplasmic

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SOCS7 (171-270/581aa)
• Target: SOCS7
• Molecular Weight: 60 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NAP4; NCKAP4; SOCS7_HUMAN; SOCS7; SOCS-7; Nck, Ash and phospholipase C gamma-binding protein; Nck-associated protein 4 (NAP-4); SOCS6; SOCS4_HUMAN; SOCS4; SOCS-4; Suppressor of cytokine signaling 7 (SOCS-7);

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebellum (Mouse) Lysate at 40 ug, Lane 3: Testis (Mouse) Lysate at 40 ug, Lane 4: Stomach (Mouse) Lysate at 40 ug, Lane 5: Placenta (Mouse) Lysate at 40 ug, Lane 6: Cerebrum (Rat) Lysate at 40 ug, Lane 7: Cerebellum (Rat) Lysate at 40 ug, Lane 8: Testis (Rat) Lysate at 40 ug, Lane 9: HepG2 (Human) Cell Lysate at 30 ug, Lane 10: Hela (Human) Cell Lysate at 30 ug, Lane 11: MCF-7 (Human) Cell Lysate at 30 ug, Lane 12: HL60 (Human) Cell Lysate at 30 ug, Lane 13: Molt-4 (Human) Cell Lysate at 30 ug, Primary: Anti-SOCS7 (orb313242) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 60 kD.

Tissue/Cell: Mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-SOCS7 Polyclonal Antibody, Unconjugated (orb313242) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SOCS7

UniProt

O14512