SOCS7 Rabbit Polyclonal Antibody

• Catalog Number: orb313242
• Category: Antibodies
• Description: SOCS7 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SOCS7 (171-270/581aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 63 kDa
• Target: SOCS7
• UniProt ID: Q8WXH5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Alternative names: NAP 4; NAP 4 Fragment; NAP4; Nck Ash and phospholi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebellum (Mouse) Lysate at 40 ug, Lane 3: Testis (Mouse) Lysate at 40 ug, Lane 4: Stomach (Mouse) Lysate at 40 ug, Lane 5: Placenta (Mouse) Lysate at 40 ug, Lane 6: Cerebrum (Rat) Lysate at 40 ug, Lane 7: Cerebellum (Rat) Lysate at 40 ug, Lane 8: Testis (Rat) Lysate at 40 ug, Lane 9: HepG2 (Human) Cell Lysate at 30 ug, Lane 10: Hela (Human) Cell Lysate at 30 ug, Lane 11: MCF-7 (Human) Cell Lysate at 30 ug, Lane 12: HL60 (Human) Cell Lysate at 30 ug, Lane 13: Molt-4 (Human) Cell Lysate at 30 ug, Primary: Anti-SOCS7 (orb313242) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 60 kD.

Tissue/Cell: Mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-SOCS7 Polyclonal Antibody, Unconjugated (orb313242) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.