SNAI2 Rabbit Polyclonal Antibody

• Catalog Number: orb11382
• Category: Antibodies
• Description: SNAI2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Slug (5-120/268aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 30 kDa
• Target: SNAI2
• UniProt ID: O43623
• RRID: AB_10751898
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: SLUG; Neural crest transcription factor Slug; Prot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-SNAI2 antibody (orb11382), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Heart (Rat) Lysate at 30 ug, Lane 2: Liver (Rat) Lysate at 30 ug, Primary: Anti-SNAI2/SLUG (orb11382) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 30kD, Observed band size: 30kD.

Sample: Lane 1: Mouse Breast tissue lysates, Lane 2: Mouse Placenta tissue lysates, Lane 3: Human MDA-MB-231 cell lysates, Lane 4: Human U2OS cell lysates, Primary: Anti-SNAI2 (orb11382) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kDa, Observed band size: 34 kDa.

Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Human MDA-MB-231 cell lysates, Primary: Anti-SNAI2 (orb11382) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kDa, Observed band size: 34 kDa.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-SNAI2 Polyclonal Antibody, Unconjugated (orb11382) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.