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Catalog Number | orb1257165 |
---|---|
Category | Antibodies |
Description | SMYD5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant protein of human SMYD5 |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 48kDa |
Target | SMYD5 |
UniProt ID | Q6GMV2 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | SMYD5, NN8-4AG, RAI15, RRG1, ZMYND23 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using SMYD5 antibody (orb1257165). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human kidney using SMYD5 antibody (orb1257165).
Immunofluorescence analysis of A549 cells using SMYD5 antibody (orb1257165). Blue: DAPI for nuclear staining.
IF, IH, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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