SMYD4 Antibody

• Catalog Number: orb1258200
• Category: Antibodies
• Description: SMYD4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 90kDa
• Target: SMYD4
• UniProt ID: Q8IYR2
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: SET and MYND domain-containing protein 4, 211-, SM
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SMYD4 antibody (orb1258200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunofluorescence analysis of MCF-7 cells using SMYD4 antibody (orb1258200). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 1 ug SMYD4 antibody (orb1258200). Western blot was performed from the immunoprecipitate using SMYD4 antibody (orb1258200) at a dilution of 1:500. Detection: ECL Enhanced Kit. Exposure time: 20s.