Smoothelin Rabbit Polyclonal Antibody

• Catalog Number: orb158429
• Category: Antibodies
• Description: Smoothelin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Primate, Rabbit
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Smoothelin (851-917/917aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 100 kDa
• Target: SMTN
• UniProt ID: P53814
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: FLJ35168; FLJ35365; FLJ35620; FLJ38597; Smoothelin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Human MCF-7 cell lysates, Lane 2: Human K562 cell lysates, Lane 3: Human Jurkat cell lysates, Primary: Anti-Smoothelin (orb158429) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100 kDa, Observed band size: 115 kDa.

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Smoothelin) Polyclonal Antibody, Unconjugated (orb158429) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.