Smooth muscle alpha-actin

• Catalog Number: orb18863
• Category: Antibodies
• Description: Goat polyclonal antibody to ACTA2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 42
• Target: Smooth muscle alpha-actin
• Entrez: 59
• Protein Sequence: EEEDSTALVC
• RRID: AB_10753000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Alternative names: Anti-a actin antibody, anti-AAT6 antibody, anti-AC
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: Successful IHC-P data obtained from a previous batch. An anonymous customer found positive results in IHC-P on Pig heart tissue.WB: Approx 45kDa band observed in Human Duodenum lysates (calculated MW of 42.0kDa according to NP_001604.1). Recommended concentration: 0.1-0.3 μg/ml.Experiment Notes: Immunofluorescence: see publication McIntire RH et al., Placenta. 2008 Jul;29(7):631-8. Epub 2008 Jun 5. (PMID: 18538388).
• Expiration Date: 12 months from date of receipt.

References

Nagy, Bence M. et al. Importance of kynurenine in pulmonary hypertension Am J Physiol Lung Cell Mol Physiol, 313, L741-L751 (2017)

PubMed: 28705908

0.3 µg/ml staining of Human (A) and (0.5 ug/ml) Mouse (B) and Rat (C) Duodenum lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.