α-Smooth Muscle Actin Antibody

SKU: orb1294387

Description

Rabbit polyclonal antibody to alpha Smooth Muscle Actin

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: Western Blot 1:1000-1:3000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:400 Immunohistochemistry (Frozen) 1:100-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Target: α-Smooth Muscle Actin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Anti-α Smooth Muscle Actin antibody at 1/2000 dilution, Lysates at 35 µg per lane, This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294387 overnight at 4°C.

Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: α Smooth Muscle Actin antibody (orb1294387).

Frozen sectioned for immunohistochemical analysis. Sample: Frozen section. Red: α Smooth Muscle Actin antibody. Catalog: orb1294387 at 1-200.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294387 anti-α Smooth Muscle Actin antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294387 anti-α Smooth Muscle Actin antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling α Smooth Muscle Actin with orb1294387 at 1/200.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling α Smooth Muscle Actin with orb1294387 at 1/200.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling α Smooth Muscle Actin with orb1294387 at 1/200.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling α Smooth Muscle Actin with orb1294387 at 1/300.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling α Smooth Muscle Actin with orb1294387 at 1/300.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling α Smooth Muscle Actin with orb1294387 at 1/300.

Quick Database Links

Gene Symbol

α-Smooth Muscle Actin