Smgc Antibody

• Catalog Number: orb1248875
• Category: Antibodies
• Description: Smgc Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, ICC
• Reactivity: Mouse
• Immunogen: The immunogen for this antibody is: C-KLEPKYENPTNGS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 65kDa and 70kDa in Mouse fetal Lung lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.4kDa according to NP_945121.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunocytochemistry: Positive staining of select epithelial cells in the murine Submanibular gland at E18. Recommended concentration 4ug/ml. Data provided by Everest Grant winner Melinda Larsen, State University of New York, Albany, NY.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Smgc
• UniProt ID: Q6JHY2
• NCBI: NP_945121.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Smgc, submandibular gland protein C, 2310010P21Rik
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 65kDa and 70kDa in Mouse fetal Lung lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.4kDa according to NP_945121.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunocytochemistry: Positive staining of select epithelial cells in the murine Submanibular gland at E18. Recommended concentration 4ug/ml. Data provided by Everest Grant winner Melinda Larsen, State University of New York, Albany, NY.
• Expiration Date: 12 months from date of receipt.

orb1248875 (4 ug/ml) staining of Mouse SubMandibular Gland cells at E18. Nuclear counterstain with DAPI in green.