Smgc Antibody

SKU: orb1248875

Description

Smgc Antibody

Images & Validation

• Tested Applications: ELISA, ICC
• Reactivity: Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 65kDa and 70kDa in Mouse fetal Lung lysates after 1µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.4kDa according to NP_945121.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunocytochemistry: Positive staining of select epithelial cells in the murine Submanibular gland at E18. Recommended concentration 4µg/mL. Data provided by Everest Grant winner Melinda Larsen, State University of New York, Albany, NY.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KLEPKYENPTNGS
• Target: Smgc
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Smgc, submandibular gland protein C, 2310010P21Rik, DXImx49e, Muc19, Sfc21, OTTMUSP00000022832, neonatal submandibular gland protein C

orb1248875 (4 ug/ml) staining of Mouse SubMandibular Gland cells at E18. Nuclear counterstain with DAPI in green.

Quick Database Links

Gene Symbol

Smgc

UniProt

Q6JHY2

RefSeq (Protein):NP_945121.1