SMARCAL1 Antibody

• Catalog Number: orb1823781
• Category: Antibodies
• Description: SmarcAL1 (SWI/SNF-related matrix-associated Actin-dependent regulator of chromatin subfamily A-like protein 1), also known as HARP (hepA-related protein) or HHARP, is a 954 amino acid member of the SWI/SNF family of helicase and ATPase proteins. Localized to the nucleus, SmarcAL1 is a ubiquitously expressed protein that functions in ATP-dependent nucleosomeremodeling activities. SmarcAL1 contains one conserved C-terminal SNF2 domain, one helicase ATP-binding domain and two HARP domains. Defects in the gene encoding SmarcAL1 are the cause of Schimke immuno-osseous dysplasia (SIOD), an autosomal recessive disorder characterized by renal dysfunction, spondyloepiphyseal dysplasia and T cell immunodeficiency.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-SMARCAL1-1C4
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: Recombinant full-length human SMARCAL1 protein was used as the immunogen for the SMARCAL1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This SMARCAL1 antibody is available for research use only.
• UniProt ID: Q9NZC9
• Storage: Aliquot the SMARCAL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human kidney carcinoma tissue with SMARCAL1 antibody (clone PCRP-SMARCAL1-1C4). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with SMARCAL1 antibody (clone PCRP-SMARCAL1-1C4). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using SMARCAL1 antibody (clone PCRP-SMARCAL1-1C4) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.