SMAD6 Rabbit Polyclonal Antibody

SKU: orb500485

Description

SMAD6 Rabbit Polyclonal Antibody

Research Area

Cardiovascular Research, Cell Biology, Cell Signaling, Cytoplasmic, DNA and RNA Research, Intracellular Signaling Proteins, SARS /COVID-19, Transcription Factors

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SMAD6 (351-450/469aa)
• Target: SMAD6
• Molecular Weight: 54 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SMAD Family Member 6; MAD Homolog 6; MADH6; MAD, Mothers Against Decapentaplegic Homolog 6(Drosophila); Mothers Against Decapentaplegic, Drosophila, Homolog Of, 6; SMAD, Mothers Against DPP Homolog 6(Drosophila); Mothers Against Decapentaplegic Homolog 6; SMAD, Mothers Against DPP Homolog 6; Mothers Against DPP Homolog 6; HsT17432; SMAD 6; HSMAD6; AOVD2; MADH7; SMAD6_HUMAN;

Paraformaldehyde-fixed, paraffin embedded (mouse uterus tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SMAD6) Polyclonal Antibody, Unconjugated (orb500485) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

SMAD6

UniProt

O43541