Smad4 Rabbit Polyclonal Antibody

SKU: orb526556

Description

Smad4 Rabbit Polyclonal Antibody

Research Area

Apoptosis, Cancer Research, Cardiovascular Research, Cell Biology, Cell Cycle, Cell Signaling, Cytoplasmic, DNA and RNA Research, Hypoxia, Intracellular Signaling Proteins, Transcription Factors, Tumor Suppressors

Images & Validation

• Tested Applications: WB
• Dilution range: WB=1:500-2000
• Reactivity: Human
• Predicted Reactivity: Bovine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Smad4 (1-100/552aa)
• Target: SMAD4
• Molecular Weight: 60 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Mothers against decapentaplegic homolog 4; (Small) Mothers Against Decapentaplegic; Deleted in Pancreatic Carcinoma; Deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; DPC 4; DPC4; hSMAD4; JIP; MAD homolog 4; MAD mothers against decapentaplegic Drosophila homolog 4; MAD mothers against decapentaplegic homolog 4; MADH 4; MADH4; Med; Medea; Mothers against decapentaplegic homolog 4; Mothers against DPP homolog 4; OTTHUMP00000163548; Smad 4; SMAD family member 4; SMAD mothers against DPP homolog 4.

Sample: Lane 1: Human K562 cell lysates, Lane 2: Human Hela cell lysates, Lane 3: Human Jurkat cell lysates, Lane 4: Human HL-60 Cell Lysates, Primary: Anti-Smad4 (orb526556) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kDa, Observed band size: 60 kDa.

Sample: Siha (Human) Cell Lysate at 30 ug, HL-60 (Human) Cell Lysate at 30 ug, Primary: Anti-Smad4 (orb526556) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 63 kD.

Quick Database Links

Gene Symbol

SMAD4

UniProt

Q13485