Smad4 Antibody

• Catalog Number: orb251580
• Category: Antibodies
• Description: Smad4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Smad4 (526-552aa EIHLHRALQLLDEVLHTMPIADPQPLD), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 60439 MW
• UniProt ID: Q13485
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Mothers against decapentaplegic homolog 4;MAD homo
• Note: For research use only
• Application notes: WB: The detection limit for Smad4 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis using Anti-SMAD4 Picoband antibody.Lane 1:Rat Brain Tissue;2:Mouse Brain Tissue;3:Rat Skeletal Muscle Tissue;4:Mouse Skeletal Muscle Tissue;5:U87 Cell;6:Human Placenta Tissue;7:HT1080 Cell;8:HELA Cell;9:NEURO Cell.