SMAD4 antibody

• Catalog Number: orb214216
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SMAD4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SMAD4. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SMAD4
• Entrez: 4089, 17128, 50554
• UniProt ID: P97471, O70437, Q13485
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti DPC4 antibody, anti MADH4 antibody, anti Moth
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SMAD4 expression in A2780 (A), H1792 (B), mouse spleen (C), rat spleen (D) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 70 kD)

Immunohistochemical analysis of SMAD4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of SMAD4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.