SMAD4 antibody

• Catalog Number: orb18328
• Category: Antibodies
• Description: Goat polyclonal antibody to SMAD4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IP
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:32000
• Conjugation: Unconjugated
• MW: 60.4
• Target: SMAD4 / MADH4
• Entrez: 4089
• Protein Sequence: HTMPIADPQPLD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti MADH4 antibody, anti SMAD4 antibody, anti JIP
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60-65kDa band seen in mouse NIH-3T3 cell lysate. Recommended for use at 0.5-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18328 staining (0.5ug/ml) of NIH-3T3 cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.