SMAD4 Antibody

• Catalog Number: orb1243942
• Category: Antibodies
• Description: SMAD4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD4.
• Concentration: batch dependent
• Dilution range: SMAD4 antibody can be used for detection of SMAD4 by ELISA at 1:312500. SMAD4 antibody can be used for detection of SMAD4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 60 kDa
• Target: SMAD4
• UniProt ID: Q13485
• NCBI: NP_005350
• Storage: For short periods of storage (days) store at 4°C. For longer periods of storage, store SMAD4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: SMAD4, JIP, DPC4, MADH4, MYHRS
• Note: For research use only
• Application notes: SMAD4 antibody can be used for detection of SMAD4 by ELISA at 1:312500. SMAD4 antibody can be used for detection of SMAD4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Expiration Date: 12 months from date of receipt.

Antibody used in WB on Human HepG2 at 1.25 ug/ml.

Antibody used in WB on Human U2OS at 1:1000.

Antibody used in WB on Human U2OS.