SMAD3-S208 Antibody

• Catalog Number: orb1927597
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB24758
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 48081 Da
• Target: This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from human SMAD3.
• UniProt ID: P84022
• NCBI: NP_001138574.1, NP_001138576.1, NP_001138575.1, NP_005893.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mothers against decapentaplegic homolog 3, MAD hom
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

SMAD3 Antibody (S208) western blot analysis in HepG2 and mouse C2C12 cell line lysates (35 ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).

Confocal immunofluorescent analysis of SMAD3-S208 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

SMAD3-S208 Antibody flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

SMAD3-S208 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMAD3-S208 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.