SMAD3-S208 Antibody

• Catalog Number: orb1261942
• Category: Antibodies
• Description: SMAD3-S208 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Gallus, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from human SMAD3.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 48 kDa
• Target: SMAD3
• UniProt ID: P84022
• NCBI: P84022
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Mothers against decapentaplegic homolog 3, MAD hom
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in HepG2 and mouse C2C12 cell line lysates (35 ug/lane).

SMAD3-S208 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of SMAD3-S208 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.