SMAD3 Antibody

• Catalog Number: orb1261259
• Category: Antibodies
• Description: SMAD3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human SMAD3 (NP_005893.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 48kDa
• Target: SMAD3
• UniProt ID: P84022
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: SMAD3, LDS3, LDS1C, MADH3, JV15-2, HSPC193, HsT174
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of U-251MG cells, using SMAD3 antibody (orb1261259) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat ovary using SMAD3 antibody (orb1261259) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat islets of langerhans using SMAD3 antibody (orb1261259) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human mammary cancer using SMAD3 antibody (orb1261259) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using SMAD3 antibody (orb1261259) at dilution of 1:100. Blue: DAPI for nuclear staining.