Smad3 (phospho-Thr179) antibody

• Catalog Number: orb313112
• Category: Tools
• Description: Smad3 (phospho-Thr179) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Thr179 (PE(p-T)PP)
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1ug/Test, WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 48 kDa
• Target: phospho-Smad3 (Thr179)
• UniProt ID: P84022
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Smad3 (phospho T179); p-Smad3 (phospho T179); DKFZ
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry of Hela.Primary Antibody (green line): Rabbit Anti-phospho-Smad3 (Thr179) antibody; Dilution:1μg /10^6 cells.

ICC analysis of Hela cell using phospho-Smad3 (Thr179) polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of Mouse adrenal gland using phospho-Smad3 Thr179 Polyclonal Antibody at 1:500 dilution.

IHC-P analysis of Rat brain using phospho-Smad3 Thr179 Polyclonal Antibody at 1:500 dilution.

IHC-P analysis of human skin cancer using phospho-Smad3 Thr179 Polyclonal Antibody at 1:200 dilution.