Smad2 (phospho-Thr220) antibody

• Catalog Number: orb769031
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Smad2 (phospho-Thr220)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Smad2 around the phosphorylation site of Thr220. AA range:186-235
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-SMAD2 antibody, anti-MADH2 antibody, anti-MAD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of HeLa cells, using Smad2 (Phospho-Thr220) Antibody. The picture on the right is blocked with the phospho peptide.