Smad2 (Phospho-Thr220) Rabbit Polyclonal Antibody

SKU: orb14987

Description

This is a rabbit polyclonal antibody targeting Smad2. It is unconjugated and suitable for use in IF, IHC, WB applications. The antibody is reactive with human, mouse, rat samples. Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:500 - 1:1000; IHC: 1:50 - 1:200); IF: /IC 1:100 - 1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide-KLH. Peptide sequence around phosphorylation site of threonine 220 (P-E-T(p)-P-P) derived from Human Smad2
• Target: Smad2
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Concentration: 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

JV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2

Quick Database Links

Gene Symbol

Smad2

UniProt

Q15796