Smad2 (phospho-S465) antibody

• Catalog Number: orb304594
• Category: Antibodies
• Description: Rabbit polyclonal antibody against Smad2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S465 of human SMAD2 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SMAD2
• Entrez: 4087, 29357, 17126
• UniProt ID: Q15796, Q62432, O70436
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-hMAD-2 antibody, anti-HsMAD2 antibody, anti-J
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SMAD2 (Phospho-S465) expression in HepG2 (A) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 60 kD)

Immunohistochemical analysis of SMAD2 (Phospho-S465) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.