Smad2 (phospho-Ser465/467) antibody

• Catalog Number: orb6979
• Category: Antibodies
• Description: Smad2 (phospho-Ser465/467) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human SMAD2 around the phosphorylation site of Ser465/467 (CS(p-S)M(p-S))
• Concentration:

  1mg/ml

• Dilution range: WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1ug/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Purity: Affinity purified by Protein A
• Conjugation: Unconjugated
• MW: 58 kDa
• Target: Phospho-Smad2 (Ser465 + Ser467)
• UniProt ID: Q15796
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Smad2(Phospho S465 + S467); phospho-Smad2(p-Ser465
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhang, Hongjun et al. Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced activation of primary hepatic stellate cells Int J Mol Med, 42, 279-289 (2018)

PubMed: 29620144

Western blot analysis of Mouse Muscle Lysate using Smad2 (phospho-Ser465-467) antibody

Western blot analysis of 7 different samples using Smad2 (phospho-Ser465/467) antibody Observed band size: 60 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain)

Paraformaldehyde-fixed, paraffin embedded (Mouse testis)

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min

Blank control (black line): Hela Primary Antibody (green line) - Dilution: 1ug/Test Secondary Antibody (white blue line) - Dilution: 0.5ug/Test Isotype control (orange line): Normal Rabbit IgG