SMAD2 Antibody

• Catalog Number: orb546274
• Category: Antibodies
• Description: SMAD2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human SMAD2 recombinant protein (Position: E83-Q264).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 60 kDa
• UniProt ID: Q15796
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Mothers against decapentaplegic homolog 2; MAD hom
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-SMAD2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of SMAD2 using anti-SMAD2 antibody.Lane 1:human placenta tissue;2:rat liver tissue;3:mouse testicular tissue;4:mouse heart tissue;5:mouse lung tissue;6:mouse lung tissue.

IF analysis of SMAD2 using anti-SMAD2 antibody. SMAD2 was detected in immunocytochemical section of HELA cells.

IHC analysis of SMAD2 using anti-SMAD2 antibody.SMAD2 was detected in paraffin-embedded section of human colon cancer tissues.

IHC analysis of SMAD2 using anti-SMAD2 antibody.SMAD2 was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of SMAD2 using anti-SMAD2 antibody.SMAD2 was detected in paraffin-embedded section of mouse brain tissues.

IHC analysis of SMAD2 using anti-SMAD2 antibody.SMAD2 was detected in paraffin-embedded section of rat brain tissues.