Smad2 antibody

• Catalog Number: orb544569
• Category: Antibodies
• Description: Smad2 antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 9A3
• Tested applications: FC, ICC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant human Smad2 protein, around N-terminal 1-200aa
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:50-200, ICC=1:100, Flow-Cyt=2ug/Test, IHC-F=1:50-200, IF=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52 kDa
• Target: Smad2
• UniProt ID: Q15796
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Mothers against decapentaplegic homolog 2; SMAD 2;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry of A431.Primary Antibody (green line): Rabbit Anti-Smad2 antibody; Dilution:1μg /10^6 cells.

ICC analysis of A549 cell using Smad2 monoclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of rat kidney using Smad2 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of mouse brain using Smad2 Polyclonal Antibody at 1:200 dilution.

Western blot:Testis (Mouse);A431(Human) Cell;HepG2(Human) Cell;HL60(Human) Cell;Jurkat(Human) Cell;Primary:Anti-Smad2 at 1:1000 dilution.