Smac Rabbit Polyclonal Antibody

SKU: orb500576

Description

Smac Rabbit Polyclonal Antibody

Research Area

Apoptosis, Cell Biology, Cell Cycle, Metabolism, Mitochondrial, Mitochondrial Markers

Images & Validation

• Tested Applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Smac (151-239/239aa)
• Target: DIABLO
• Molecular Weight: 21 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Smac/Diablo; 0610041G12Rik; DBLOH_HUMAN; DBOH; DBOH; diablo; Diablo homolog(Drosophila); Diablo homolog(Drosophila); Diablo homolog; Diablo homolog Drosophila; Diablo homolog mitochondrial; Diablo homolog mitochondrial precursor; DIABLO S; DIABLO S; DIABLOS; Direct IAP binding protein with low pI; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial; Mitochondrial Smac protein; Second Mitochondria Derived Activator of Caspase; Second mitochondria-derived activator of caspase; SMAC 3; smac; Smac protein; SMAC3.

Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug Lane 2: Testis (Mouse) Lysate at 40 ug Primary: Anti-alpha smooth muscle Actin at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 19 kD.

Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Smac) polyclonal Antibody, Unconjugated (orb500576) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

DIABLO

UniProt

Q9NR28