SLC6A12/BGT1 Antibody

SKU: orb1248508

Description

SLC6A12/BGT1 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in Human Brain lysates from several regions (calculated MW of 69.4kDa according to NP_003035.3). Recommended concentration: 1-3µg/mL. An additional band of unknown identity was also consistently observed at 27kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: CLDGSAGRNFGPSPT
• Target: SLC6A12 / BGT1
• Molecular Weight: Approx 70kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

SLC6A12, solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12, BGT-1, BGT1, FLJ38727, GAT2, Na(+)/Cl(-) betaine/GABA transporter, OTTHUMP00000115358, OTTHUMP00000237543, OTTHUMP00000237583, gamma-aminobutyric acid transporter, sodium- and chloride-dependent betaine transporter

orb1248508 (1 ug/ml) staining of Human Olfactory bulb lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248508 (10 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

SLC6A12 / BGT1

UniProt

P48065

RefSeq (Protein):NP_003035.3