SLC5A1 Rabbit Polyclonal Antibody

SKU: orb11364

Description

SLC5A1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SGLT1 (501-600/665aa)
• Target: SLC5A1
• Molecular Weight: 73 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

D22S675; High affinity sodium glucose cotransporter 1; GLT1; GLT-1; High affinity sodium glucose cotransporter; Human Na+/glucose cotransporter 1; Na(+)/glucose cotransporter 1; NAGT; SGLT 1; SGLT1; Sodium glucose cotransporter 1; Sodium/glucose cotransporter 1; Solute carrier family 5(sodium/glucose cotransporter) member 1; Solute carrier family 5 member 1.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SGLT1) Polyclonal Antibody, Unconjugated (orb11364) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Kidney Lysates, Lane 2: Human 293T cell Lysates, Lane 3: Human HepG2 cell Lysates, Primary: Anti-SLC5A1 (orb11364) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 73kDa, Observed band size: 70kDa.

Quick Database Links

Gene Symbol

SLC5A1

UniProt

P13866