SLC35D2 Rabbit Polyclonal Antibody

• Catalog Number: orb317805
• Category: Antibodies
• Description: SLC35D2 Rabbit Polyclonal Antibody
• Target: SLC35D2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Porcine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Immunogen: KLH conjugated synthetic peptide derived from mouse SLC35D2 (251-326/326aa)
• MW: 37 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Hfrc; HFRC1; Homolog of Fringe connection protein
• Note: For research use only

Tissue/Cell: mouse kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-SLC35D2 Polyclonal Antibody, Unconjugated (orb317805) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Sample: liver (Mouse) Lysate at 40 ug, Primary: Anti-SLC35D2 (orb317805) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 37kD, Observed band size: 35kD.